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The dual agonist mechanism provides robust glycemic control and significant weight reduction, with clinical trials demonstrating A1C reductions of 1.92.4% and weight loss of approximately 1522% of body weight in non-diabetic adults with obesity (SURMOUNT-1 trial), with more modest weight loss typically observed in patients with type 2 diabetes

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Towards this end, we used HeLa cells in which endogenous PDS5B is silenced by an siRNA targeting the 3-UTR of the PDS5B mRNA and complemented with an ectopically expressed, siRNA resistant form of HA-tagged PDS5B WT or PDS5B 17A